cd25 bc96 antibody  (Thermo Fisher)

Journal: iScience

Article Title: Exploring the dynamics and influencing factors of CD4 T cell activation using single-cell RNA-seq

doi: 10.1016/j.isci.2023.107588

Figure Lengend Snippet:

Article Snippet: CD25-PE (clone: BC96) , eBioscience , Cat #12-0259-42; RRID: AB_1659682.

Techniques: Recombinant, SYBR Green Assay, Real-time Polymerase Chain Reaction, Software

Journal: Cells

Article Title: The Composition of Adipose-Derived Regenerative Cells Isolated from Lipoaspirate Using a Point of Care System Does Not Depend on the Subject’s Individual Age, Sex, Body Mass Index and Ethnicity

doi: 10.3390/cells12010030

Figure Lengend Snippet: Primary antibodies used for flow cytometry in the present study.

Article Snippet: CD25 , BC96 , IgG1, kappa , PE , eBioscience/Thermo Fisher , 12-0259-42.

Techniques: Flow Cytometry

Journal: Cells

Article Title: The Composition of Adipose-Derived Regenerative Cells Isolated from Lipoaspirate Using a Point of Care System Does Not Depend on the Subject’s Individual Age, Sex, Body Mass Index and Ethnicity

doi: 10.3390/cells12010030

Figure Lengend Snippet: Combinations of surface markers/primary antibodies (M) and conjugates (C) used for flow cytometry in the present study.

Article Snippet: CD25 , BC96 , IgG1, kappa , PE , eBioscience/Thermo Fisher , 12-0259-42.

Techniques: Flow Cytometry, Cytometry

Journal: Cells

Article Title: The Composition of Adipose-Derived Regenerative Cells Isolated from Lipoaspirate Using a Point of Care System Does Not Depend on the Subject’s Individual Age, Sex, Body Mass Index and Ethnicity

doi: 10.3390/cells12010030

Figure Lengend Snippet: Combination of surface markers/primary antibodies used for flow cytometry, the respective cell type as defined in the literature, the corresponding reference to the literature, and the results of flow cytometry (mean, standard deviation, standard error of the mean, minimum, median and maximum of the relative numbers of cells (cells/cells in per cent) that were positive for the tested combination of surface markers/primary antibodies).

Article Snippet: CD25 , BC96 , IgG1, kappa , PE , eBioscience/Thermo Fisher , 12-0259-42.

Techniques: Flow Cytometry, Standard Deviation

Journal: Cells

Article Title: The Composition of Adipose-Derived Regenerative Cells Isolated from Lipoaspirate Using a Point of Care System Does Not Depend on the Subject’s Individual Age, Sex, Body Mass Index and Ethnicity

doi: 10.3390/cells12010030

Figure Lengend Snippet: Results ( p values) of the statistical analysis (univariate ANOVA with fixed factors and covariates) of the data shown in Table 7 . p values < 0.05 are given boldface.

Article Snippet: CD25 , BC96 , IgG1, kappa , PE , eBioscience/Thermo Fisher , 12-0259-42.

Techniques:

Journal: Frontiers in Immunology

Article Title: B cells from anti-thyroid antibody positive, infertile women show hyper-reactivity to BCR stimulation

doi: 10.3389/fimmu.2022.1039166

Figure Lengend Snippet: Different naive and memory B cell ratio in levothyroxine treated and ATA+, euthyroid, infertile patients Prevalence of naive and memory B cell subsets (A) , B cell memory subpopulations (B) and CD25 + B cell subpopulations (C) HC: healthy controls (n=12); HT: Hashimoto’s thyroiditis, H1: hypothyroid patients with HT before treatment (n=14); H2: euthyroid patients with HT, after levothyroxine-treatment, (n=12); HIE: HT, infertile, euthyroid patients (n=14); NSw: non-switched; Sw: switched, DN: double negative memory B cells. Differences between the groups and within the B cell subpopulations were assessed with two-way ANOVA and Tukey’s post hoc test except H1-H2 comparisons where a mixed-effects analysis and the Sidak’s multiple comparisons tests were used because these groups represent the same individuals in a repeated sampling before and during treatment. Bars represent the mean ± standard deviation (SD) of percentages of lymphocyte subpopulation in each group. *p < 0.05, **p < 0.01.

Article Snippet: PBMCs (8 x 10 6 cells) were stained with the appropriate combination of fluorochrome-conjugated anti-human antibodies in 100 μl of media in order to differentiate B lymphocyte subsets: CD5 PerCP (clone UCHT2), CD19 PE (clone SJ25C1), IgD APC (clone IA6-2), CD25 BV421 (clone BC96) (all from Sony Biotechnology), CD27 PE-Cy7(clone M-T271, BioLegend).

Techniques: Sampling, Standard Deviation

Journal: Frontiers in Immunology

Article Title: B cells from anti-thyroid antibody positive, infertile women show hyper-reactivity to BCR stimulation

doi: 10.3389/fimmu.2022.1039166

Figure Lengend Snippet: Basal calcium level is elevated in B cells from ATA+, euthyroid, infertile patients Baseline median fluorescence intensity of Fluo4 in B cell subpopulations (A) , CD25- (C) and CD25+ (E) B cells. Cross tabulation shows the p values when comparing patient groups without the subpopulation-based grouping of B cells (B) , CD25- (D) and CD25+ cells (F) . HC: healthy controls (n=12); HT: Hashimoto’s thyroiditis, H1: hypothyroid patients with HT before treatment (n=14); H2: euthyroid patients with HT, after levothyroxine-treatment, (n=12); HIE: HT, infertile, euthyroid patients (n=14); NSw: non-switched; Sw: switched, DN: double negative memory B cells. Differences between the groups and within the B cell subpopulations were assessed with two-way ANOVA and Tukey’s post hoc test except H1-H2 comparisons, where a mixed-effects analysis and the Sidak’s multiple comparisons test were used because these groups represent the same individuals in a repeated sampling before and during treatment. Data are presented as follows: middle line represents the median, dot represents the mean, box: interquartile range, whiskers: min-max. *p < 0.05, **p < 0.01; Significant p values are red, non-significant p values are written in blue.

Article Snippet: PBMCs (8 x 10 6 cells) were stained with the appropriate combination of fluorochrome-conjugated anti-human antibodies in 100 μl of media in order to differentiate B lymphocyte subsets: CD5 PerCP (clone UCHT2), CD19 PE (clone SJ25C1), IgD APC (clone IA6-2), CD25 BV421 (clone BC96) (all from Sony Biotechnology), CD27 PE-Cy7(clone M-T271, BioLegend).

Techniques: Fluorescence, Sampling

Journal: Frontiers in Immunology

Article Title: B cells from anti-thyroid antibody positive, infertile women show hyper-reactivity to BCR stimulation

doi: 10.3389/fimmu.2022.1039166

Figure Lengend Snippet: Naive B cells show higher Ca 2+ signal upon BCR stimulation in ATA+ euthyroid, infertile patients Calcium flux kinetic data of naive B, naive CD25- and naive CD25+ B cells (A) maximum value, (B) ending value, (C) time to reach maximum, (D) time to first 50% value, (E) time from first 50% to maximum, (F) time from maximum to second 50% value, (G) slope at the first 50% value, (H) slope at second 50% value, (I) area under curve (AUC) value. HC: healthy controls (n=12); HT: Hashimoto’s thyroiditis, H1: hypothyroid patients with HT before treatment (n=14); H2: euthyroid patients with HT, after levothyroxine-treatment, (n=12); HIE: HT, infertile, euthyroid patients (n=14); NSw: non-switched; Sw: switched, DN: double negative memory B cells. The kinetic data were analyzed by the FACSKin software. Differences between the groups were assessed with two-way ANOVA and Tukey’s post hoc test except H1-H2 comparisons where a mixed-effects analysis and the Tukey’s multiple comparisons test were used because these groups represent the same individuals in a repeated sampling before and during treatment. Data are depicted as individual values, middle line represents the mean, whiskers are set to standard deviation (SD) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: PBMCs (8 x 10 6 cells) were stained with the appropriate combination of fluorochrome-conjugated anti-human antibodies in 100 μl of media in order to differentiate B lymphocyte subsets: CD5 PerCP (clone UCHT2), CD19 PE (clone SJ25C1), IgD APC (clone IA6-2), CD25 BV421 (clone BC96) (all from Sony Biotechnology), CD27 PE-Cy7(clone M-T271, BioLegend).

Techniques: Software, Sampling, Standard Deviation

Journal: Frontiers in Immunology

Article Title: B cells from anti-thyroid antibody positive, infertile women show hyper-reactivity to BCR stimulation

doi: 10.3389/fimmu.2022.1039166

Figure Lengend Snippet: Memory B cells of ATA+, euthyroid, infertile patients have increased calcium flux upon BCR activation Selected calcium flux kinetic data of memory B cell subsets: Non-switched (NSw) memory B cells, CD25- and CD25+ NSw cells: (A) maximum value, (B) ending value, (C) area under curve (AUC) value. Immunoglobulin class-switched (Sw) memory B cells, CD25- and CD25+ Sw cells: (D) maximum value, (E) ending value, (F) area under curve (AUC) value. CD27-, IgD- double-negative (DN) memory B cells, CD25- and CD25+ DN cells: (G) maximum value, (H) ending value, (I) area under curve (AUC) value. HC: healthy controls (n=12); HT: Hashimoto’s thyroiditis, H1: hypothyroid patients with HT before treatment (n=14); H2: euthyroid patients with HT, after levothyroxine-treatment, (n=12); HIE: HT, infertile, euthyroid patients (n=14). The kinetic data were analyzed by the FACSKin software. Differences between the groups were assessed with two-way ANOVA and Tukey’s post hoc test except H1-H2 comparisons where a mixed-effects analysis and the Tukey’s multiple comparisons test were used because these groups represent the same individuals in a repeated sampling before and during treatment. Data are depicted as individual values, middle line represents the mean, whiskers are set to standard deviation (SD). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: PBMCs (8 x 10 6 cells) were stained with the appropriate combination of fluorochrome-conjugated anti-human antibodies in 100 μl of media in order to differentiate B lymphocyte subsets: CD5 PerCP (clone UCHT2), CD19 PE (clone SJ25C1), IgD APC (clone IA6-2), CD25 BV421 (clone BC96) (all from Sony Biotechnology), CD27 PE-Cy7(clone M-T271, BioLegend).

Techniques: Activation Assay, Software, Sampling, Standard Deviation